A reference-based protein degradation assay without global translation inhibitors
نویسندگان
چکیده
منابع مشابه
A reference-based protein degradation assay without global translation inhibitors.
Although it is widely appreciated that the use of global translation inhibitors, such as cycloheximide, in protein degradation assays may result in artefacts, these inhibitors continue to be employed, owing to the absence of robust alternatives. We describe here the promoter reference technique (PRT), an assay for protein degradation with two advantageous features: a reference protein and a gen...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2017
ISSN: 0021-9258
DOI: 10.1074/jbc.m117.814236